| 质粒名称: | pHLuc Control |
|---|---|
| 出品公司: | Thermo Fisher Scientific |
| 目录编号: | 141091 |
| 存储实验室: | Jeak Ling Ding |
|
载体骨架基本信息 |
|
| 载体名称: | pCDNA3.1 |
| 载体出品公司: | Thermo Fisher Scientific |
| 空载体大小: | 4606 bp |
| 完整质粒大小: | 8968bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc / pHLuc Control / Control |
| 物种来源: | H. sapiens (human), M. musculus (mouse) |
| 插入大小: | 4374bp |
| 启动子: | CMV |
| 克隆方法: | 未知 |
| 5’测序引物 | T7 forward (TTAATACGACTCACTATAGGG) |
| 3’测序引物 | BGH reverse (TAGAAGGCACAGTCGAGG) |
|
文献引用方法 |
|
| 材料和方法部分: | pHLuc Control was a gift from Jeak Ling Ding (Addgene plasmid # 141091 ; http://n2t.net/addgene:141091 ; RRID:Addgene_141091) |
| 参考文献部分: | pHLuc, a Ratiometric Luminescent Reporter for in vivo Monitoring of Tumor Acidosis. Ong TT, Ang Z, Verma R, Koean R, Tam JKC, Ding JL. Front Bioeng Biotechnol. 2020 May 8;8:412. doi: 10.3389/fbioe.2020.00412. eCollection 2020. 10.3389/fbioe.2020.00412 PubMed 32457886 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHLuc Control 8968 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION pH-stable bioluminescent reporter that serves as the control
counterpart of pHLuc; utilizes eGFP instead of SEP.
ACCESSION .
VERSION .
KEYWORDS pHLuc Control
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8968)
AUTHORS Ong TT, Ang Z, Verma R, Koean R, Tam JKC, Ding JL
TITLE pHLuc, a Ratiometric Luminescent Reporter for in vivo Monitoring of
Tumor Acidosis.
JOURNAL Front Bioeng Biotechnol. 2020 May 8;8:412. doi:
10.3389/fbioe.2020.00412. eCollection 2020.
PUBMED 32457886
REFERENCE 2 (bases 1 to 8968)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8968
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(23..40)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 29..253
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 299..727
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(386..405)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 596..617
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(736..756)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 741..1070
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 921..1056
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 983..1002
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 1310..1431
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1347..1366)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1401..1420
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(1480..1496)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1480..1496)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1493..1515)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1504..1520
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1528..1558)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1573..1594
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1711..1728)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1882..2470)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1962..1981)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2641..3501)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3264..3283
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3502..3606)
/gene="bla"
/label=AmpR promoter
primer_bind complement(3681..3700)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 3872..4251
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4252..4455
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4406..4426
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 4500..4519
/label=T7
/note="T7 promoter, forward primer"
promoter 4500..4518
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 5279..5785
/codon_start=1
/product="NanoLuc(R) luciferase"
/label=Nluc
/note="human codon-optimized"
/translation="FTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIV
LSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTP
NMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRL
CERILA"
CDS 6485..6538
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 6545..7144
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(6545..6564)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 7041..7061
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 7146..7719
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(7313..7330)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 7540..7559
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 7724..8440
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 7724..8440
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(7769..7790)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(8030..8049)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 8377..8398
/label=EGFP-C
/note="EGFP, forward primer"
ORIGIN
1 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
61 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
121 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
181 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
241 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
301 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
361 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
421 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
481 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
541 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
601 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
661 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
721 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
781 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
841 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
901 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
961 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
1021 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
1081 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcgcgg
1141 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
1201 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
1261 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
1321 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
1381 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
1441 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
1501 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
1561 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
1621 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
1681 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
1741 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
1801 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
1861 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
1921 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
1981 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
2041 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
2101 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
2161 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
2221 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
2281 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
2341 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
2401 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
2461 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
2521 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
2581 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
2641 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
2701 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
2761 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
2821 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
2881 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
2941 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
3001 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
3061 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
3121 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
3181 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
3241 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
3301 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
3361 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
3421 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
3481 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
3541 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
3601 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
3661 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
3721 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
3781 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
3841 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
3901 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
3961 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
4021 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
4081 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
4141 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
4201 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
4261 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
4321 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
4381 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
4441 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
4501 aatacgactc actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg
4561 agctcggatc cactagtcca gtgtggtgga attcatggtg agcaagggcg aggagctgat
4621 caaggagaac atgagaagca agctgtacct ggaaggcagc gtgaacggcc accagttcaa
4681 gtgcacccac gaaggggagg gcaagcccta cgagggcaag cagaccaaca ggatcaaggt
4741 ggtggaggga ggccccctgc cgttcgcatt cgacatcctg gccacccact ttatgtacgg
4801 gagcaaggtg ttcatcaagt accccgccga cctccccgat tattttaagc agtccttccc
4861 tgagggcttc acatgggaga gagtcatggt gttcgaagac gggggcgtgc tgaccgccac
4921 ccaggacacc agcctccagg acggcgagct catctacaac gtcaaggtca gaggggtgaa
4981 cttcccagcc aacggccccg tgatgcagaa gaaaacactg ggctgggagc ccagcaccga
5041 gaccatgtac cccgctgacg gcggcctgga aggcagatgc gacaaggccc tgaagctcgt
5101 gggcgggggc cacctgcacg tcaacttcaa gaccacatac aagtccaaga aacccgtgaa
5161 gatgcccggc gtccactacg tggaccgcag actggaaaga atcaaggagg ccgacaacga
5221 gacctacgtc gagcagtacg agcacgctgt ggccagatac tccaacctgg gcggaggctt
5281 cacactcgaa gatttcgttg gggactggcg acagacagcc ggctacaacc tggaccaagt
5341 ccttgaacag ggaggtgtgt ccagtttgtt tcagaatctc ggggtgtccg taactccgat
5401 ccaaaggatt gtcctgagcg gtgaaaatgg gctgaagatc gacatccatg tcatcatccc
5461 gtatgaaggt ctgagcggcg accaaatggg ccagatcgaa aaaattttta aggtggtgta
5521 ccctgtggat gatcatcact ttaaggtgat cctgcactat ggcacactgg taatcgacgg
5581 ggttacgccg aacatgatcg actatttcgg acggccgtat gaaggcatcg ccgtgttcga
5641 cggcaaaaag atcactgtaa cagggaccct gtggaacggc aacaaaatta tcgacgagcg
5701 cctgatcaac cccgacggct ccctgctgtt ccgagtaacc atcaacggag tgaccggctg
5761 gcggctgtgc gaacgcattc tggcgaggca cgagctgatc aaggagaaca tgagaagcaa
5821 gctgtacctg gaaggcagcg tgaacggcca ccagttcaag tgcacccacg aaggggaggg
5881 caagccctac gagggcaagc agaccaacag gatcaaggtg gtggagggag gccccctgcc
5941 gttcgcattc gacatcctgg ccacccactt tatgtacggg agcaaggtgt tcatcaagta
6001 ccccgccgac ctccccgatt attttaagca gtccttccct gagggcttca catgggagag
6061 agtcatggtg ttcgaagacg ggggcgtgct gaccgccacc caggacacca gcctccagga
6121 cggcgagctc atctacaacg tcaaggtcag aggggtgaac ttcccagcca acggccccgt
6181 gatgcagaag aaaacactgg gctgggagcc cagcaccgag accatgtacc ccgctgacgg
6241 cggcctggaa ggcagatgcg acaaggccct gaagctcgtg ggcgggggcc acctgcacgt
6301 caacttcaag accacataca agtccaagaa acccgtgaag atgcccggcg tccactacgt
6361 ggaccgcaga ctggaaagaa tcaaggaggc cgacaacgag acctacgtcg agcagtacga
6421 gcacgctgtg gccagatact ccaacctggg cggaggcatg gacgagctgt acaaggcggc
6481 cgctgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggcccttc
6541 cgggatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
6601 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
6661 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
6721 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
6781 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
6841 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
6901 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
6961 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
7021 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
7081 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
7141 ctgaacccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
7201 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
7261 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
7321 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
7381 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
7441 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
7501 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
7561 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
7621 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
7681 cacggggacg tggttttcct ttgaaaaaca cgatgataat gtgatggtga gcaagggcga
7741 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
7801 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
7861 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
7921 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
7981 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
8041 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
8101 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
8161 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
8221 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
8281 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
8341 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
8401 cgccgccggg atcactctcg gcatggacga gctgtacaag gatatttccg ggggggtctt
8461 tacacttgaa gatttcgtcg gggattggag acaaactgcc ggttataatc ttgatcaggt
8521 gcttgagcaa gggggggtga gctccctttt tcaaaacctg ggtgtatctg tcacccctat
8581 ccaaaggatt gtgcttagcg gtgaaaatgg tctcaaaatc gacatccacg tgatcatccc
8641 atacgagggt cttagtggag atcagatggg gcagatagag aagattttca aagttgttta
8701 ccctgtcgac gaccaccatt tcaaagtaat tctgcactac ggaaccctcg tcatagacgg
8761 ggtcacaccc aacatgatag attactttgg gcgaccctat gaagggatag cagtattcga
8821 cggcaaaaag ataaccgtga caggcaccct gtggaatgga aacaaaatta tagatgagcg
8881 cctgatcaac cctgacggtt ctttgttgtt cagggttaca atcaatggtg tcacaggctg
8941 gaggctctgt gagcgcatcc tggcttaa
//
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